T. Parpala-sparman et al., Closed-circuit organ perfusion technique for gene transfer into the lungs.An experimental trial on farm pigs, EUR J CL IN, 31(3), 2001, pp. 264-271
Citations number
34
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research General Topics
Background In an attempt to develop gene therapy for lung diseases, we have
explored a closed-circuit surgical perfusion method for gene transfer into
the lung.
Materials and methods For gene transfer we used a replication defective typ
e 5 adenovirus carrying the E. coli beta -galactosidase gene as a reporter
gene. The middle lobe of the right lung of eight young farm pigs was perfus
ed in vivo via thoracotomy for up to 60 min with the viral solution. The ge
ne transfer was performed using a closed-circuit organ perfusion method in
vivo. The efficiency of gene transfer was assessed visually by analysis of
histologic sections after X-gal, PAS and immunohistochemical stainings.
Results The lung perfusion resulted in transgene expression in the alveolar
epithelial cells, capillary endothelial cells, airway epithelial cells and
alveolar macrophages of the lung examined seven days after perfusion.
Conclusion The present results suggest that operatively performed closed-ci
rcuit warm lung perfusion method may be used for gene transfer in treatment
of diseases that have pulmonary manifestations.