Reduced intracellular survival of Helicobacter pylori vacA mutants in comparison with their wild-types indicates the role of VacA in pathogenesis

Citation
Am. Petersen et al., Reduced intracellular survival of Helicobacter pylori vacA mutants in comparison with their wild-types indicates the role of VacA in pathogenesis, FEMS IM MED, 30(2), 2001, pp. 103-108
Citations number
43
Categorie Soggetti
Immunology
Journal title
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
ISSN journal
09288244 → ACNP
Volume
30
Issue
2
Year of publication
2001
Pages
103 - 108
Database
ISI
SICI code
0928-8244(200103)30:2<103:RISOHP>2.0.ZU;2-1
Abstract
The vacuolating cytotoxin VacA of Helicobacter pylori plays an important bu t yet unknown role in pathogenesis. We studied the impact of the vacuolatin g cytotoxin on Ii. pylori invasion of and survival within AGS cells (human gastric cell line derived from an antral adenocarcinoma). Isogenic vacA and cagA mutants were constructed in a wild-type clinical isolate H. pylori. A F4. An H. pylori VacA-deficient mutant, AF4(vacA::kan). was cultured in sig nificantly lower numbers fi om AGS cells after 24 h incubation with gentami cin added to the culture medium than were the type I wild-type strain AF4 ( P<0.03) and an isogenic cagA mutant (P<0.01). Complementation of the AF4 va cA mutant with broth culture supernatant from wild-type AF4 improved the in tracellular survival of the vacA mutant. We conclude that H. pylori's vacuo lating cytotoxin improves the intracellular survival of H. pylori within AG S cells, suggesting the role of the vacuolating cytotoxin in II. pylori pat hogenesis. (C) 2001 Federation of European Microbiological Societies. Publi shed by Elsevier Science B.V. All rights reserved.