Chromosomal location of ribosomal DNA (rDNA), (GATA)(n) and (TTAGGG)(n) telomeric repeats in the neogastropod Fasciolaria lignaria (Mollusca : Prosobranchia)

Authors
Vitturi, R Colomba, MS Gianguzza, P Pirrone, AM
Citation
R. Vitturi et al., Chromosomal location of ribosomal DNA (rDNA), (GATA)(n) and (TTAGGG)(n) telomeric repeats in the neogastropod Fasciolaria lignaria (Mollusca : Prosobranchia), GENETICA, 108(3), 2000, pp. 253-257
Citations number
38
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENETICA
ISSN journal
00166707 → ACNP
Volume
108
Issue
3
Year of publication
2000
Pages
253 - 257
Database
ISI
SICI code
0016-6707(2000)108:3<253:CLORD(>2.0.ZU;2-4
Abstract
This paper reports on a successful application of fluorescent in situ hybri dization (FISH) with three repetitive DNA probes (ribosomal DNA (rDNA), (GA TA)(n) and (TTAGGG)(n)) in the chromosomes of Fasciolaria lignaria (Mollusc a: Prosobranchia: Neogastropoda). rDNA FISH consistently identified four ch romosome pairs per spread in the three examined specimens. The telomeric se quence (TTAGGG)(n) hybridized with termini of all chromosomes. GATA FISH re vealed abundant, dispersed minisatellite regions which were not associated to the XY sex-determining mechanism as indicated by the absence of a Y spec ific pattern of labelling.