Distribution of the transposable element Minos in the genus Drosophila

We analyzed 28 species or the genus Drosophila for the presence of the Tc1- like transposable element Minos using Southern blot hybridization under hig h stringency conditions. The Minos transposon was found in members of both the Drosophila and the Sophophora subgenus showing a distribution that is w ider if compared to other well-studied Drosophila transposons such as the P element, hobo and mariner. The presence of Mines-hybridizing sequences was discontinuous in the Sophophora subgenus, especially in the melanogaster s pecies group. Using the Polymerase Chain Reaction we amplified a portion co rresponding to the putative Mines transposase from different Drosophila spe cies. Cloning and sequence analysis of randomly selected Mines copies from D. mojavensisis, D. saltans and D. willistoni supports the idea that event( s) of horizontal transfer may have contributed to the spreading of this tra nsposon in the Drosophila genus.