Analysis of trace elements released from hornblende between pH 6.5 and 7.5
in the presence of Arthrobacter sp. shows that Fe, Ni, V, Mn, and, to a les
ser extent, Co are preferentially released into solution relative to bacter
ia-free experiments. This enhanced release into solution could be due to co
ntributions from the slightly lowered pH, the presence of low molecular wei
ght organic acids (LMWOAs), or the presence of a catecholate siderophore in
experiments with bacteria. The best explanation for enhanced metal release
is siderophore complexation at the mineral surface followed by release to
solution. However, the relative rates of metal release to solution in these
experiments do not strictly follow the trend predicted by the relative ord
ering of metal hydrolysis, which might be predicted for siderophore-promote
d dissolution. For some of these metals, release to solution is fast initia
lly in biotic experiments, but concentrations in solution reach a steady st
ate value or decrease with time as the bacteria cell numbers increase expon
entially. Lack of enhanced release to solution for some metals and decrease
s in release rate with time for others may be explained by uptake into bact
eria. Many of the metals predicted to strongly complex with siderophore (in
cluding Al, Ti, Fe, Cu) are heavily taken up into cellular material. The re
lative ordering of organic ligand-element complexation may therefore partia
lly explain the relative ordering of uptake of trace metals and rare earth
elements into cell material. Fractionation of heavy rare earth elements tak
en up into cellular material is also very strong, and increases from Ho to
Lu. Strong fractionation in uptake of some elements by bacteria may create
biological signatures either in the mineral substrate or in any mineral pre
cipitates associated with the cellular material.