ELUCIDATION OF THE STRUCTURE OF TALINOLOL METABOLITES IN MAN - DETERMINATION OF TALINOLOL AND HYDROXYLATED TALINOLOL METABOLITES IN URINE AND ANALYSIS OF TALINOLOL IN SERUM

The objective of this study was to determine the structure of talinolo l metabolites formed and the amounts excreted in urine. Talinolol meta bolites in urine were identified by comparing their HPLC retention tim es and their GC-MS profile with those of previously characterized refe rence compounds. The metabolites were quantified by HPLC with a normal -phase silica column, a single chloroform extraction and UV detection. Less than 1% of an administered dose was found in urine as hydroxylat ed talinolol. Other metabolites could be excluded. A sensitive method to determine talinolol in serum and a simple method for analysis of ta linolol in urine are described. These methods were found to be precise and accurate for the measurement of talinolol in samples obtained fro m patients during chronic talinolol treatment as well as from healthy volunteers after a single dose of talinolol.