ULTRA-RAPID PREPARATION OF MILLIGRAM QUANTITIES OF THE PURIFIED POLYPEPTIDE-CHAINS OF HUMAN FIBRINOGEN

In order to study the epitopes in fibrin towards which monoclonal anti bodies are directed we needed the pure individual polypeptide chains o f human fibrinogen in reasonable quantity. We report here a simplified , rapid method of separation of high-purity human fibrinogen chains. F ollowing reduction and S-carboxymethylation of human fibrinogen, the s ample was injected directly onto a column of the polymeric reversed-ph ase perfusion packing POROS 20-R2, and the chains were completely reso lved in less than 3 min at a flow-rate of 10 ml/min. The capacity was equivalent to that of a similar sized conventional silica-based column . However the throughput was approximately five to ten times as high. The column was durable and robust in day-to-day use.