Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin

Authors
Allen, AG Bolitho, S Lindsay, H Khan, S Bryant, C Norton, P Ward, P Leigh, J Morgan, J Riches, H Eastty, S Maskell, D
Citation
Ag. Allen et al., Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin, INFEC IMMUN, 69(4), 2001, pp. 2732-2735
Citations number
13
Categorie Soggetti
Immunology
Journal title
INFECTION AND IMMUNITY
ISSN journal
00199567 → ACNP
Volume
69
Issue
4
Year of publication
2001
Pages
2732 - 2735
Database
ISI
SICI code
0019-9567(200104)69:4<2732:GACOAD>2.0.ZU;2-0
Abstract
A defined allelic-replacement mutant of the sly gene, encoding a thiol-acti vated cytolysin, from a European isolate of Streptococcus suis serotype 2 w as generated and characterized. Unlike the parental strain, it is nonhemoly tic, noncytotoxic for cultured macrophage-like cells, avirulent in a mouse infection model, yet only slightly attenuated in a porcine model of systemi c infection.