Circulating tumor-derived DNA may permit the early diagnosis of head and neck squamous cell carcinomas

A series of eight microsatellite loci were assayed for both loss of heteroz ygosity and new mutated alleles in 91 head and neck squamous cell carcinoma s, In 58 cases, alterations were detected and used as markers for assaying the presence of circulating tumor-derived DNA in the patients' plasma. This was unambiguously detected in 17 cases. The probability of detecting circu lating DNA was independent of tumor stage and was found to be present even in some individuals with stage I tumors. The presence of such DNA, however, could not be correlated with disease outcome or other significant clinical parameters, suggesting that it has no prognostic significance. The results indicate that circulating tumor-derived DNA could be used as a means of ea rly diagnosis of head and neck tumors. (C) 2001 Wiley-Liss, Inc.