Detection of Salmonella enteritidis in shell and liquid eggs using enrichment and plating

Detection methods using various enrichment and plating media and immunoconc entration for Salmonella enteritidis in shell and liquid eggs were evaluate d. For liquid egg samples naturally contaminated with S. enteritidis, pre-e nrichment in 225 ml of buffered peptone water with cysteine followed by sel ective enrichment in 10 mi of tetrathionate broth was the superior, resulti ng in the detection of S. enteritidis in all samples on six of the seven ty pes of selective agar substrate investigated. This enrichment procedure als o enabled detection of S. enteritidis in most of artificially inoculated sh ell egg and pasteurized liquid egg samples. (C) 2001 Elsevier Science B.V. All rights reserved.