A. Antignac et al., Polymorphism of Neisseria meningitidis penA gene associated with reduced susceptibility to penicillin, J ANTIMICRO, 47(3), 2001, pp. 285-296
We studied polymorphism of penA (which encodes penicillin-binding protein 2
) in 13 strains of Neisseria meningitidis susceptible to penicillin (pen(S)
) and 12 strains with reduced susceptibility to penicillin (pen(I)). These
strains differed in geographical origin. Serological and genetic typing sho
wed that they were highly diverse and belonged to several genetic lineages.
Restriction analysis and DNA sequencing of penA showed that all pens strai
ns had the same penA allele regardless of genetic group, whereas pen(I) str
ains harboured various penA alleles. Transformation with amplicons of penA
and genomic DNA from several pen(I) strains conferred the pen(I) phenotype
on a pen(S) strain. Thus, reduction in susceptibility to penicillin is dire
ctly related to changes in penA and analysis of penA polymorphisms could be
used as a reliable tool for characterizing meningococcal strains in terms
of their susceptibility to penicillin.