Escherichia coli MutS,L modulate RuvAB-dependent branch migration between diverged DNA

This study examines the interaction between Escherichia coli MutS,L and E. coli RuvAB during E. coli Reck-promoted strand exchange. RuvAB is a branch migration complex that stimulates heterologous strand exchange, Previous st udies indicate that RuvAB increases the rate at which heteroduplex products are formed by RecA, that RuvA and RuvB are required for this stimulation, and that RuvAB does not stimulate homologous strand exchange. This study in dicates that MutS,L inhibit the formation of full-length heteroduplex DNA b etween M13-fd DNA in the presence of RuvAB, such that less than 2% of the l inear substrate is converted to product. Inhibition depends on the time at which MutS,L are added to the reaction and is strongest when MutS,L are add ed during initiation. The kinetics of the strand exchange reaction suggest that MutS,L directly inhibit RuvAB-dependent branch migration in the absenc e of Reck The inhibition requires the formation of base-base mismatches and ATP utilization; no effect on RuvAB-promoted strand exchange is seen if an ATP-deficient mutant of MutS (MutS501) is included in the reaction instead of wild-type MutS. These results are consistent with a role for MutS,L in maintaining genomic stability and replication fidelity.