Measurement of one-bond H-1(alpha)-C-13(alpha) couplings in backbone-labelled proteins

Authors
Giesen, AW Bae, LC Barrett, CL Chyba, JA Chaykovsky, MM Cheng, MC Murray, JH Oliver, EJ Sullivan, SM Brown, JM Dahlquist, FW Homans, SW
Citation
Aw. Giesen et al., Measurement of one-bond H-1(alpha)-C-13(alpha) couplings in backbone-labelled proteins, J BIOM NMR, 19(3), 2001, pp. 255-260
Citations number
26
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOMOLECULAR NMR
ISSN journal
09252738 → ACNP
Volume
19
Issue
3
Year of publication
2001
Pages
255 - 260
Database
ISI
SICI code
0925-2738(200103)19:3<255:MOOHCI>2.0.ZU;2-1
Abstract
NMR dipole-dipole couplings between protein backbone nuclei (H-1(alpha), C- 13(alpha), N-15, H-1(N),C-13') offer enormous scope for the rapid determina tion of protein global folds. Here, we show that measurement of one-bond sp littings in the protein backbone is facilitated by use of protein that is s electively isotopically enriched only in the backbone atoms. In particular, H-1(alpha)-C-13(alpha) couplings can be measured simply and with high sens itivity by use of conventional heteronuclear single quantum correlation (HS QC) techniques.