Chiral separation of several thiobarbiturates on a cellulose tris(4-methylbenzoate) phase under reversed-phase conditions

The chiral separation of several thiobarbiturates on a tris(4-methylbenzoat e) phase (Chiralcel OJ-R, 15 x 0.46 cm and Chiralcel OJ-R 15 x 0.20 cm) und er reversed-phase conditions is described. The separation properties of a n ormal sized and a newly developed narrow-bore column were compared. The inf luence of buffer and organic modifiers on enantioselectvity was investigate d. The three mobile phases used were water/acetonitrile (70.30, v/v), water /acetonitrile/methanol (50:25:25, v/v), and water/acetonitrile/methanol (55 :15:30, v/v). Of seven thiobarbiturates investigated, five have been resolv ed. The values of alpha (1.11-1.85) and R-s (0.71-5.75) have been reported.