Historically, malaria was a significant cause of morbidity and mortality th
roughout the western United States, and Anopheles freeborni Aitken was thou
ght to be the vector west of the Continental Divide. In 1989, Anopheles her
msi Barr & Guptavanij was described and subsequently found to be an effecti
ve laboratory vector of Plasmodium. The adults of these two species are mor
phologically indistinguishable, and therefore polymerase chain reaction was
used to analyze the DNA from 48 mosquitoes collected in Arizona and Colora
do (identified morphologically as An. freeborni). All specimens were identi
fied as An. hermsi. This was the first report of An. hermsi in Arizona and
Colorado and indicated that this Anopheles species historically may have be
en a malaria vector in these two western states.