Demonstration of the Rb-1 lipopolysaccharide core structure in Salmonella strains with the monoclonal antibody M105

The lipopolysaccharide (LPS) from 42 strains representing 19 Salmonella ser ogroups was differentiated into characteristic ladder-like profiles by SDS- PAGE analysis, The core-specific antibody M105 (Ra, Rb-1 and Rb-2) was used in an immunoblot assay of SDS-PAGE-separated LPS molecules. The M105 antib ody bound to the R-type LPS of 18 of the 20 Salmonella strains tested, The results demonstrate that S. enterica serotype Godesberg, S, Adelaide (one o f two strains), S. Milwaukee, S. Niarembe, S. Bere and S. Arizonae (serogro up 63) have an atypical LPS core structure which is Rb-1 type.