Twelve dextran-producing Leuconostoc strains were characterized according t
o their antibiotic susceptibilities, carbohydrate fermentation profiles, su
crase activity patterns and plasmid content. All the strains tested were re
sistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and
could be separated into two groups based on whether or not they could ferm
ent melibiose and raffinose. Six Leuconostoc strains possessed plasmids and
many produced unique sucrase activity patterns in polyacrylamide gels. The
se data will aid in distinguishing among physiologically similar dextran-pr
oducing leuconostocs, frequently used in research and industry.