QUANTITATIVE-ANALYSIS OF COMPONENTS OF THE COLOR ADDITIVES D-AND-C RED-NOS-27 AND RED-NOS-28 (PHLOXINE-B) BY THIN-LAYER CHROMATOGRAPHY AND VIDEO DENSITOMETRY

Authors
WRIGHT PR RICHFIELDFRATZ N RASOOLY A WEISZ A
Citation
Pr. Wright et al., QUANTITATIVE-ANALYSIS OF COMPONENTS OF THE COLOR ADDITIVES D-AND-C RED-NOS-27 AND RED-NOS-28 (PHLOXINE-B) BY THIN-LAYER CHROMATOGRAPHY AND VIDEO DENSITOMETRY, JPC. Journal of planar chromatography, modern TLC, 10(3), 1997, pp. 157-162
Citations number
8
Categorie Soggetti
Chemistry Analytical
Journal title
JPC. Journal of planar chromatography, modern TLC → ACNP
ISSN journal
09334173
Volume
10
Issue
3
Year of publication
1997
Pages
157 - 162
Database
ISI
SICI code
0933-4173(1997)10:3<157:QOCOTC>2.0.ZU;2-P
Abstract
A simple and rapid TLC - videodensitometric method has been developed for the in situ quantification either of lower halogenated subsidiary colors (LHSC) or of the ethyl ester (EE) in multiple dye samples on th e same analytical TLC plate. The results obtained by this method were compared with those obtained by an indirect TLC - spectrophotometric m ethod and those obtained by HPLC. The total time for the TLC - videode nsitometric assay of five standards and four samples applied to each p late was 45 min or less. This method for the determination of LHSC and EE in D&C Red Nos. 27 and 28 was found to be applicable for use in ro utine batch-certification analysis.