VERIFICATION OF THIN-LAYER CHROMATOGRAPHY THEORY .2. THE CHARACTERISTICS OF THE 2ND, EQUILIBRIUM STAGE OF CHROMATOGRAPHIC-SEPARATION

Study of the factors which influence the progress of a chromatographic separation provide data which enable better understanding of the proc ess. The properties of the liquid-crystal detector have been used to d isplay changes in the distribution of spot concentration in the second stage of the chromatographic process (in the mobile phase range Z(f) > 20 mm). Estimates were made of the influence on spot broadening of t he type of chromatographic system, the concentration distribution at t he origin, R-F values, and the duration of the chromatographic profess . The results obtained from the investigation were used to verify Bele nky's dynamic theory of thin-layer chromatography.