Intrathecal magnesium sulfate attenuates algogenic behavior and spinal amino acids release after kainic acid receptor activation in rats

Activation of N-methyl-D-asparate (NMDA) receptor and non-NMDA classes of g lutamate receptors play a key role in spinal nociceptive processing. Using with a lumbar intrathecal (IT) catheter and a loop dialysis catheter in lig htly anesthetized (1% isoflurane) rats, the effect of IT pre-treatment with magnesium sulfate(100, 300 or 500 mug) on IT kainic acid (KA: 1 mug; non-N MDA receptor agonist) evoked amino acids (AAs) release and corresponding be havior was examined. IT KA produced significant increases (mean +/- SD of % baseline concentration) in dialysate concentrations of aspartate (424 +/- 88%), glutamate (241 +/- 35%) and taurine (398 +/- 58%). IT pre-treatment w ith MgSO4 resulted in a dose-dependent suppression of the evoked algogenic behavior and aspartate release. These data suggest that activation of spina l KA receptors provides a powerful stimulus for secondary spinal excitatory AAs release and corresponding appearance of pain behavior. The regulation of this release by magnesium suggests the possible role of this divalent ca tion in regulating this excitatory effect of non-NMDA receptor activation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.