Rapid modeling of two-dimensional multi-photon excitation microscopic imaging through turbid medium

A rapid model to study two-dimensional (2-D) multi-photon excitation (MPE) fluorescence microscopic imaging through turbid medium has been presented. This model combines traditional Monte Carlo method with 2-D point spread fu nction (PSF). Planar fluorescent object was used to calculate the 2-D PSF, which avoided the simulation of point scanning in the direct Monte Carlo me thod. In the meantime, this rapid model has generalized program logic and n eed only one-step Monte Carte calculation for the simulations of single. tw o- and three-photon excitation fluorescence microscopic imaging. Experiment al results showed that the rapid model using convolution operation was much faster than the direct Monte Carlo method. Similar computation efficiency was achieved for different order of MPE. This model provides high potential in the simulation of MPE fluorescence imaging. (C) 2001 Published by Elsev ier Science B.V.