R. Marsou et al., Relationship of Staphylococcal isolates in a Moroccan hospital by comparing phenotypical and genotypical tests, PATH BIOL, 49(2), 2001, pp. 109-114
The relationship of Staphylococcus isolates was determined among a collecti
on of 26 clinical strains at the Centre Hospitalier Universitaire de Rabat.
These isolates originated principally from blood culture and wounds. In or
der to affirm the clonal origins of these isolates, six phenotype (biotype,
antibiotype, serotype, phage type), and genotype (random amplified polymor
phic DNA, pulsed field gel electrophoresis) methods were used. Biotyping, a
ntibiotyping, phage and serotyping were generally not sufficient while many
isolates remained nonphage typable. Random amplified polymorphic DNA analy
sis used in epidemiological typing seemed suitable for S. epidermidis and S
. haemolyticus. However, rigorous standardization will be needed to assure
reliable results. Pulsed field gel electrophoresis discriminated more effic
iently than random amplification polymorphic DNA analysis. This study attes
ts to the suitability of two or more methods in combination for typing Stap
hylococcus isolates. (C) 2001 Editions scientifiques et medicales Elsevier
SAS.