Evidence from action and fluorescence spectra that UV-induced violet-blue-green fluorescence enhances leaf photosynthesis

Citation
Sv. Mantha et al., Evidence from action and fluorescence spectra that UV-induced violet-blue-green fluorescence enhances leaf photosynthesis, PHOTOCHEM P, 73(3), 2001, pp. 249-256
Citations number
46
Categorie Soggetti
Biochemistry & Biophysics
Journal title
PHOTOCHEMISTRY AND PHOTOBIOLOGY
ISSN journal
00318655 → ACNP
Volume
73
Issue
3
Year of publication
2001
Pages
249 - 256
Database
ISI
SICI code
0031-8655(200103)73:3<249:EFAAFS>2.0.ZU;2-6
Abstract
We assessed the contribution of W-induced violet-blue-green leaf fluorescen ce to photosynthesis in Poa annua, Sorghum halepense and Nerium oleander by measuring UV-induced fluorescence spectra (280-380 nm excitation, 400-550 nm emission) from leaf surfaces and determining the monochromatic UV action spectra for leaf photosynthetic O-2-evolution, Peak fluorescence emission wavelengths from leaf surfaces ranged from violet (408 nm) to blue (448 nn) , while excitation peaks for these maxima ranged from 333 to 344 nm, Action spectra were developed by supplementing monochromatic radiation from 280 t o 440 nm, in 20 nm increments, to a visible nonsaturating background of 500 mu mol m(-2) s(-1) photosynthetically active radiation and measuring photo synthetic O-2-evolution rates. Photosynthetic rates tended to be higher wit h the 340 nm supplement than with higher or lower wavelength UV supplements . Comparing photosynthetic rates with the 340 nm supplement to those with t he 400 nm supplement, the percentage enhancement in photosynthetic rates at 340 nm ranged from 7.8 to 9.8%. We suspect that 340 nm UV improves photosy nthetic rates via fluorescence that provides violet-blue-green photons for photosynthetic energy conversion because (1) the peak excitation wavelength (340 nm) for violet-blue-green fluorescence from leaves was also the most effective UV wavelength at enhancing photosynthetic rates, and (2) the magn itude of photosynthetic enhancements attributable to supplemental 340 nm UV was well correlated (R-2 = 0.90) with the apparent intensity of 340 nm UV- induced violet-blue-green fluorescence emission from leaves.