R. Ferris et al., Leaf area is stimulated in Populus by free air CO2 enrichment (POPFACE), through increased cell expansion and production, PL CELL ENV, 24(3), 2001, pp. 305-315
The effects of free-air CO2 enrichment (FACE) on leaf growth in Populus, wa
s studied. For the first time in field conditions, both the production and
expansion of leaf cells were shown to be sensitive to atmospheric carbon di
oxide. Leaf area expansion rate and final leaf size were stimulated under F
ACE for three species (Populus x euramericana (I-214), P. nigra (Jean Pourt
et) and P. alba (2AS-11), with the largest effect observed for P. x euramer
icana (61%). In this species and in P. nigra, both epidermal cell size and
cell number were increased, whereas for P. alba, only cell production was i
ncreased in FACE. Two findings suggest that changes in the cell wall may be
important in explaining larger leaf cells in FACE: (i) Leaf cell wall exte
nsibility of rapidly growing leaves increased in all species in FACE; and (
ii) an increase in xyloglucan endotransglycosylase activity, a cell wall-lo
osening enzyme, was increased in FACE and associated with leaf growth rate.
The results suggest that the mechanisms by which FACE promotes leaf growth
differ, depending on species. Despite this, increases in final leaf size p
rovide an important component driving increased biomass accumulation in POP
FACE, during this first year of rapid growth, prior to canopy closure. The
question as to whether these effects are the result of a direct response to
CO2, or are driven indirectly through substrate availability remains unres
olved, although evidence from the literature suggests that the latter mecha
nism is most likely.