KERATINOCYTE-LYMPHOCYTE INTERACTION IN CUTANEOUS T-CELL LYMPHOMA - MODULATION OF KERATINOCYTE ANTIGEN MY7 BY A SOLUBLE FACTOR PRODUCED BY T-LYMPHOCYTES

Objective: To test the hypothesis that the modulation of My7 antigen i n the basal keratinocytes is directly related to the effect of dermal lymphocyte infiltrate of epidermotropic cutaneous T-cell lymphoma (CTC L). Design: In vitro study with reconstituted skin model. Setting: Dep artment of Dermatology of Universitary Hospital, Nantes, France. Patie nts: Lymphocytes extracted from 11 skin samples with lesions of epider motropic CTCL (mycosis fungoides, stages Ila to IV) and 6 skin samples with lesions of atopic dermatitis (control population) together with the supernatants of these infiltrating lymphocytes were incubated with normal reconstituted skin samples either alone or in the presence of interferon alfa-2a (10(2) IU/mL). Moreover, normal peripheral blood mo nonuclear cells of 7 patients and 4 controls were incubated with re co nstituted skin. Intervention: None. Main Outcome Measures: None. Resul ts: Ten of 11 samples of lymphocytes extracted from CTCL and 7 of 11 o f their supernatants inhibited partially or completely My7 expression by basal cells. No inhibition was noted for lymphocytes extracted from inflammatory skin or their supernatants. Addition of interferon alfa- 2a in a culture medium of extracted lymphocytes or their supernatants blocked inhibition of My7 expression by keratinocytes in 8 of 10 recon stituted skin samples. No abrogation of My7 expression was noted with peripheral mononuclear cells. Conclusions: Our in vitro study demonstr ated a direct and specific interaction between the tumor infiltrate of CTCL and keratinocytes. Moreover, this interaction appeared to be clo sely associated with a soluble factor produced by the tumor T-cell inf iltrate and was at least partially blocked by interferon alfa-2a.