Modulation of HSP70 and HSP90 expression by sodium salicylate and aspirin in fish cell line CHSE-214

Nonsteroidal anti-inflammatory drugs (NSAIDs) such as sodium salicylate, as pirin and indomethacin have been reported to activate the heat shock transc ription factor (HSF) without enhancing the expression of the heat shock pro teins (HSPs). We investigated the effects of NSAIDs on the heat shock-induc ed HSP expression in fish cell line CHSE-214. Here we reveal that in CHSE-2 14 cells, co-exposure to sodium salicylate/aspirin and heat shock (24 degre esC) enhances and prolongs the heat shock-induced HSP70 expression presumab ly through activation of the HSF. In contrast, the heat shock-induced HSP70 expression was inhibited by these NSAIDs. Thus, sodium salicylate and aspi rin are likely to exert different effects on the heat shock-induced HSP70 a nd HSP90 expression. Indomethacin, another cyclooxygenase inhibitor, had no stimulatory or inhibitory effects on the heat shock-induced HSP70 and 90 e xpression, thereby indicating that sodium salicylate and aspirin may modula te the heat shock response via pathways not involving cyclooxygenase. Since anti-oxidants could inhibit the heat shock-induced HSP70 expression, the s timulatory effects of sodium salicylate and aspirin on the HSP70 expression are not likely due to their ability to act as anti-oxidants. Additionally, sodium salicylate and aspirin could exert synergistic effects on the HSP70 expression at lower temperatures (20-22 degreesC) that did not induce the HSP70 expression.