Direct effect of bile on colonic mucosa in alimentary induced hyperlipidemy in rats

An experimental surgical model was developed in rats after a short term ali mentary induced hyperlipidemy to study the direct effect of bile on the col onic mucosa, with regard to the cancerogenic properties of lipid rich diet. The purpose of this study was to light on the role of fatty acid alteratio n and lipid peroxidation processes of bile in the epithelial cell damage. A nimals were fed with normal (group A) and fat rich diet (group B) for 10 da ys and then bile samples were collected by the cannulation of the common bi le duct in deep anaesthesia. The circulation preserved colons of control ra ts were treated either with bile from the control or hyperlipidemic rats. T he treatment was carried out for 30 minutes. The electronmicroscopic alterations of epithelial cells (both enterocytes a nd goblet cells) caused by bile from hyperlipidemic rats were significantly greater than that of controls. Unfavourable changes of the redox state of the colonic mucosa were also det ected both in the hyperlipidemic and bile treated groups. A significant inc rease was observed in the free-SH concentration of the two bile treated gro ups against the untreated animals. The changes could be explained among oth ers by the modified bile fatty acid composition. The present study supports that the alimentary modified bile can influence the structure of the epith elium of colonic mucosa and it can be one of the inducing factor of carcino genesis.