Kinetic study on the coordination of the agents with nucleotides using Os-H-2 complex as a probe

Interaction between dGMP or its one - binding- site analogues- imidazole, N aH2PO3, Na2HPO4 and the probe [ trans - en(2)Os(eta (2)-H-2)D2O](2+) were s tudied. The results suggested that the coordination between dGMP and the pr obe was not the simple sum of the coordination of probe with imidazole and that with NaH2PO4 or Na2HPO4. The solution chemistry and the dynamics of an ticancer agents Me2SnCl2, Bu2SnCl2 competing for the binding sites of dGMP with the probe at different concentrations were studied using H-1 NMR and U V-stopped-flow instruments. Binding sites, equilibrium constants and the dy namic results of the probe and the anticancer agents with dGMP were thus ob tained. Some reaction mechanisms is also proposed.