Resolution and quantification in poorly separated peaks from capillary zone electrophoresis using three-way data analysis methods

This paper shows the use of three-way data analysis methods to improve reso lution and quantification in poorly separated peaks from capillary zone ele ctrophoresis (CZE). Second-order data (data matrices) obtained with a diode array detector (DAD) are analyzed. There is a large overlap in the electro phoretic time domain and the data do not have a strict trilinear structure. These shortcomings might hinder the mathematical resolution. However, thre e-way methods allow the pure electrophoretic profiles of the analytes to be recovered in an unknown mixture, even in the presence of uncalibrated inte rferences. Furthermore, the analyte can be quantified in unknown mixtures w ith reasonable accuracy using pure standards. As an example, ebrotidine met abolites were chosen to test the method. (C) 2001 Elsevier Science B.V. All rights reserved.