An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity

Authors
Hilden, L Eng, L Johansson, G Lindqvist, SE Pettersson, G
Citation
L. Hilden et al., An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity, ANALYT BIOC, 290(2), 2001, pp. 245-250
Citations number
31
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ANALYTICAL BIOCHEMISTRY
ISSN journal
00032697 → ACNP
Volume
290
Issue
2
Year of publication
2001
Pages
245 - 250
Database
ISI
SICI code
0003-2697(20010315)290:2<245:AACDBC>2.0.ZU;2-U
Abstract
The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phane rochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA. system this biosensor can repla ce colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same resu lt as the Somogyi-Nelson method in a less time-consuming and laborious mann er. The two methods showed about the same precision. (C) 2001 Academic Pres s.