Plant tissue-based chemiluminescence flow biosensor for glycolic acid

A novel plant tissue-based chemiluminescence (CL) biosensor for glycolic ac id combined with now injection analysis is proposed in this paper. The spin ach tissue acts as the molecular recognition element. Glycolic acid is oxid ized by oxygen under the catalysis of glycolate oxidase in the tissue colum n to produce hydrogen peroxide, which can react with luminol in the presenc e of peroxidase of spinach tissue to generate a CL signal. The CL emission intensity was linear with glycolic acid concentration in the range of 4 x 1 0(-3)-4 x 10(-6) mol/L and the detection limit was 1.3 x 10(-6) mol/L. The biosensor was stable for about 3 weeks. A complete analysis, including samp ling and washing, could be performed in 1.5 min with a relative standard de viation of 1.7%.