Mm. Sala et al., Measurement of ectoenzyme activities as an indication of inorganic nutrient imbalance in microbial communities, AQUAT MIC E, 23(3), 2001, pp. 301-311
Assessment of nutrient limitation in microbial populations in marine enviro
nments is an important topic. Existing methodologies generally demand long
incubations in an experimental assay manipulating nutrients, We suggest a n
ovel approach using the ratio between 2 ectoenzymatic activities, alkaline
phosphatase (APA) and aminopeptidase (AMA), to evaluate nutrient Limitation
of microbial communities in situ. APA is used as an indication of P limita
tion and AMA of N limitation. The ratio bypasses the use of biomass estimat
ions to calculate specific activities, which are error-prone and time-consu
ming. Our results from enrichment experiments have shown that the ratio APA
:AMA increases in P-deficient treatments, and decreases after inorganic P a
ddition. After P-deficient nutrient addition (n treatments) the ratio APA:A
MA increased to 90-230%, whereas following N+P balanced additions the ratio
decreased to 30-70% of the initial value. These results demonstrate that t
his approach is easy, quick and gives a general insight into the nitrogen v
ersus phosphorus nutrient balance of the communities without the need for b
iomass estimations or measurement of actual nutrient concentrations.