HOMOKARYOTIC MYCELIAL PELLETS OF BASIDIOM YCETES - APPLICATION TO GENETIC-ANALYSIS

An experimental haploid-diploid system suitable for genetic analysis o f Pleurotus ostreatus using homokaryotic pellets that represent the cl one offspring of individual spores has been developed. Allele segregat ion at six out of seven loci did not differ from the expected one. Sig nificant variation from the expected 1 : 1 segregation was observed at two phosphohexaisomerase loci (probably linked). The procedure elabor ated allows one (1) to significantly increase the efficiency of popula tion genetic analysis in a large group of organisms, basidium fungi; ( 2) to use these fungi as a model system in fundamental and applied res earch.