Analysis of molecular interactions of the p53-family p51(p63) gene products in a yeast two-hybrid system: Homotypic and heterotypic interactions and association with p53-regulatory factors
T. Kojima et al., Analysis of molecular interactions of the p53-family p51(p63) gene products in a yeast two-hybrid system: Homotypic and heterotypic interactions and association with p53-regulatory factors, BIOC BIOP R, 281(5), 2001, pp. 1170-1175
Citations number
37
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
p51 in the p53 tumor suppressor family, also referred to as p63, encodes mu
ltiple isoforms including p51A (TAp63 gamma) and p51B (TAp63 alpha). The p5
3 protein forms a tetramer, and its stability and activity are regulated by
molecular association with viral and cellular proteins and by biochemical
modifications. Using a yeast two-hybrid system, the p51A and p51B isoforms
were examined for homotypic and heterotypic interactions in the p53 family
proteins and for their affinity to the p53-regulatory factors. Results indi
cate a homotypic interaction dependent on the presumed oligomerization doma
in of the p51 proteins. The possibility of a weak heterotypic interaction b
etween p51 and p73 proteins was suggested, while association between p51 an
d p53 appeared improbable. Furthermore, unlike p53, the p51 proteins failed
to display an affinity to SV40 large T antigen or MDM2-family proteins. Ha
ving several features in common with p53, the p51 proteins may function in
biological processes apart from p53. (C) 2001 Academic Press.