Vm. Dirsch et Am. Vollmar, Ajoene, a natural product with non-steroidal anti-inflammatory drug (NSAID)-like properties?, BIOCH PHARM, 61(5), 2001, pp. 587-593
The inducible isoform of cyclooxygenase (COX-2) is implicated in the pathog
enesis of various inflammatory diseases as well as in carcinogenesis, espec
ially of gastrointestinal tumors. Epidemiological as well as experimental d
ata support a role for constituents of allium vegetables, such as garlic an
d onions, in the prevention of gastrointestinal cancer. Therefore, the aim
of the present study was to examine whether the garlic-derived natural prod
uct ajoene interferes with the COX-2 pathway by using lipopolysaccharide (L
PS)-activated RAW 264.7 cells as in vitro model. Ajoene was shown to dose-d
ependently inhibit the release of LPS (1 mug/mL)-induced prostaglandin E-2
in RAW 264.7 macrophages (IC50 value: 2.3 muM). This effect was found to be
due to an inhibition of COX-2 enzyme activity by ajoene (IC50 value: 3.4 m
uM). Ajoene did not reduce COX-2 expression, but rather increased LPS-induc
ed COX-2 protein and mRNA expression compared to LPS-stimulated cells only.
In the absence of LPS, however, ajoene was unable to induce COX-2. The non
-steroidal anti-inflammatory drug indomethacin was shown to act similarly i
n LPS-activated RAW 264.7 cells. These data suggest that ajoene works by a
mechanism of action similar to that attributed to non-steroidal anti-inflam
matory drugs. This finding may add a novel aspect to the biological profile
of the garlic-derived natural product ajoene which might be important for
understanding the usefulness of garlic for chemoprevention of gastrointesti
nal carcinomas. (C) 2001 Elsevier Science Inc. All rights reserved.