We cloned novel Na+-ATPase (HANA) cDNA from marine alga Heterosigma akashiw
o. The full-length HANA cDNA was 4467 bp long and coded for a 1330 amino ac
id protein with a molecular weight of 146 306. The deduced product exhibite
d around 40% identity in amino acids with Na+/K+-ATPase alpha -subunits. A
hydrophilic sequence of 285 amino acid residues that showed no homology wit
h any sequence listed in databases existed in the M7-M8 junction of HANA. T
his is the first report on the primary structure of putative Na+-transporti
ng ATPase from plant cells. (C) 2001 Elsevier Science B.V. All rights reser
ved.