Analysis of filtration kinetics of heterogeneous erythrocyte suspensions

Using the model of the passage of a single erythrocyte through a narrow cha nnel and the model of heterogeneous suspension filtration, we considered th e dependence of filtration kinetics of such suspensions at constant pressur e on the character of erythrocyte distribution in the parameters influencin g their rheological characteristics under various osmolarities of a suspend ing medium (ranging from 200 to 550 mosM). In case of the uniform suspensio n, the filtration rate reaches its stationary value quickly (for time less than 100 ms). In case of the heterogeneous suspension, the filtration trate becomes stationary in larger intervals of time (1-10 s) or does not become stationary at all. It was shown that the dependence of the suspension filt ration rates in time (filtration curves) at various medium osmolalities (u) might be helpful in obtaining the information about the spahe of erythrocy te distribution. We developed the algorithm of processing the filtration cu rves which allows us to estimate the rate of passage through a pore (w) for erythrocyte of the distribution centre and the coefficient of deviation of the distribution. The mathematical model which describes the way w depends on u makes it possible to estimate the surface area, the coefficient of os motic sensitivity, the volume, the hemoglobin content in the erythrocyte, a s well parameters, reflecting the influence of membrane rigidity and intrac ellular viscosity on w (for the cells of the distribution center). It was s hown that the presented experimental data can be explained only if we sugge st that the surface area, the coefficient of osmotic sensitivity, and the h emoglobin content of the erythrocyte are posively correlated.