High performance liquid chromatography analysis of D-ribose content in glucose zymotic fluid

Authors
Zhang, JF Wang, JG Deng, GC Yang, SH Chen, RT
Citation
Jf. Zhang et al., High performance liquid chromatography analysis of D-ribose content in glucose zymotic fluid, CHEM J CH U, 22(1), 2001, pp. 43-45
Citations number
4
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
22
Issue
1
Year of publication
2001
Pages
43 - 45
Database
ISI
SICI code
0251-0790(200101)22:1<43:HPLCAO>2.0.ZU;2-9
Abstract
The method has been put forward to analyze the content of D-ribose obtained from glucose zymotic fluid. D-ribose was determined by HPLC, on a Sugar Pa k I column, by using. a refractive index detector and a mobile phase of pur e water. We have also studied the separation conditions of sugars. It has m any advantages, such as rapid analysis, high sensitivity and high precision and good reproducibility. The recovery of D-ribose were 95.5%-104%, RSD we re less than 1.5%, the detection limit of D-Ribose was 50 ng.