A new method for analysing the ingredients of rhynchophylline and isrhyncho
phylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thi
n layer chromatographic (TLC) and the surface-enhanced raman spectroscopic(
SERS) technics is reported. The results show that the characteristic spectr
al bands of rhynchophylline can be obtained from the TLC spot with minimum
amount of rhynchophylline sample of about 2.5 mm diameter. The difference b
etween the SERS and solid spectra are found. Spectral band at 1 397 cm(-1)
was obtained wits great enhanced want, Molecule was absorbed in surface sil
ver sol by pair of electrons in -OCH3, Isrhynchophylline sample spectrum ba
nd at 1 615 cm(-1) obtained the greatest enhance. Isrhynchophylline molecul
e was absorbed in surface silver sol by pair of electron at N atom in ring
indole. An absorption model of rhynchophylline and isrhynchophylline and si
lver sol is proposed. That effective combination of TLC and SERS can be use
d to analyse the chemical ingredients with high sensitivity.