TLC-FT-SERS study on a pair isomer in Uncaria Rhynchophylla Jacks alkali

Citation
Y. Wang et al., TLC-FT-SERS study on a pair isomer in Uncaria Rhynchophylla Jacks alkali, CHEM J CH U, 22(1), 2001, pp. 46-48
Citations number
10
Categorie Soggetti
Chemistry
Journal title
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
ISSN journal
02510790 → ACNP
Volume
22
Issue
1
Year of publication
2001
Pages
46 - 48
Database
ISI
SICI code
0251-0790(200101)22:1<46:TSOAPI>2.0.ZU;2-S
Abstract
A new method for analysing the ingredients of rhynchophylline and isrhyncho phylline in Uncaria Rhynchophylla Jacks using hyphenaed high efficiency thi n layer chromatographic (TLC) and the surface-enhanced raman spectroscopic( SERS) technics is reported. The results show that the characteristic spectr al bands of rhynchophylline can be obtained from the TLC spot with minimum amount of rhynchophylline sample of about 2.5 mm diameter. The difference b etween the SERS and solid spectra are found. Spectral band at 1 397 cm(-1) was obtained wits great enhanced want, Molecule was absorbed in surface sil ver sol by pair of electrons in -OCH3, Isrhynchophylline sample spectrum ba nd at 1 615 cm(-1) obtained the greatest enhance. Isrhynchophylline molecul e was absorbed in surface silver sol by pair of electron at N atom in ring indole. An absorption model of rhynchophylline and isrhynchophylline and si lver sol is proposed. That effective combination of TLC and SERS can be use d to analyse the chemical ingredients with high sensitivity.