M. Lipp et al., Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs, EUR FOOD RE, 212(4), 2001, pp. 497-504
A qualitative screening method was validated for the detection of genetical
ly modified organisms (GMOs) in various processed food matrices (cooked mai
ze grit, infant formula, biscuits, meal of acidified soybeans). The prepare
d food matrices contained each 0%, 2%, 100% (10% instead of 100% in the cas
e of biscuits) of Roundup-Ready(R) soybeans and/or of Bt-176 maize. The met
hod was based on the detection of the introduced DNA sequences by using the
polymerase chain reaction (PCR) for amplification of DNA. Two different de
tection systems were applied: one based on the 35S promoter fragment which
is present in GM-soybeans and in GM-maize and one based on the nos terminat
or sequence which is present only in GM-soybeans. Prior to the validation s
tudy, it was demonstrated that the food matrices were homogenous and that t
here was no cross-contamination between GMO-containing and GMO-free samples
. Some laboratories had a high portion of false positive results probably d
ue to laboratory contamination whereas all others reported nearly always co
rrect data for all matrices investigated. Statistical analysis showed that
after exclusion of the outliers, an average of 97% correct results was obta
ined for non-GMO containing samples (3% false positive results) and an aver
age of 98% correct results for GMO-containing samples (2% false negative re
sults). The validated method was shown to be suitable for screening of CMOs
in processed food matrices also when extreme physical stress was applied (
e.g., heating for 45 min at 100 degreesC or 10 min at 180 degreesC) or when
multiple components were present, as in the case of biscuit preparations.