Resolution of infection by Leishmania sp. is critically dependent on activa
tion of CD4(+) T helper cells. Naive CD4(+) T helper cells are primed by de
ndritic cells which have responded to an activation signal in the periphery
. However, the role of Leishmania-infected dendritic cells in the activatio
n of an anti-leishmania immune response has not been comprehensively addres
sed. Using the highly controlled model system of bone marrow-derived dendri
tic cell infection by Leishmania mexicana cultured in vitro, we show that u
ptake of L. mexicana parasites does not result in activation of immature de
ndritic cells or secretion of IL-12. Incubation with L. mexicana promastigo
tes results in the activation of a small percentage of dendritic cells whic
h do not. appear to contain whole parasites. Activation of dendritic cells
is not suppressed by infection, since infected cells can be fully activated
on addition of activating stimuli. Therefore, uptake of intact Leishmania
mexicana parasites is not sufficient to activate dendritic cells in vitro.
We propose that these data provide a basis for interpreting the interaction
s between dendritic cells and all Leishmania sp.