Decreased antioxidant defense during replicative aging of the yeast Saccharomyces cerevisiae studied using the 'baby machine' method

Authors
Grzelak, A Skierski, J Bartosz, G
Citation
A. Grzelak et al., Decreased antioxidant defense during replicative aging of the yeast Saccharomyces cerevisiae studied using the 'baby machine' method, FEBS LETTER, 492(1-2), 2001, pp. 123
Citations number
22
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
492
Issue
1-2
Year of publication
2001
Database
ISI
SICI code
0014-5793(20010309)492:1-2<123:DADDRA>2.0.ZU;2-O
Abstract
Replicatively senescent cells of Saccharomyces cerevisiae were obtained usi ng the 'baby machine' method by immobilizing cells on CovaLinks(TM) NH2 pla tes and allowing them to divide while exchanging medium and removing daught er cells. Centrifugation in a Percoll density gradient was employed for fur ther purification of replicatively old yeast cells. Comparison of senescent cells showing more than 20 bud stars with cells from early stationary cult ure demonstrated a significant reduction of total and reduced glutathione a nd decrease of superoxide dismutase activity during replicative aging of ye ast cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.