The condition mutation cdc6-1 affects chromosome segregation in Saccharomyces cerevisiae

CDC6 is an essential gene of Saccharomyces cerevisiae involved in the initi ation of DNA replication. Interacting with ORC complex of proteins, Cdc6 pr otein has a pivotal role in loading Mcm proteins on origins of replication. Although much evidence about its function in S phase of the cell cycle is available, only a few data indicate a regulatory function of this protein i n G2/M transition phase of the cell cycle. By synchronisation with a microt ubule-destabilising drug nocodazole and Fluorescence Activating Cell Sortin g (FACS) analysis it was possible to provide evidence that CDC6 is responsi ble for a proper sequence of genetic events and accurate chromosome segrega tion during mitosis.