Characterization of the X-linked murine centrin Cetn2 gene

A multi-gene family (Cetn1, Cetn2, and Cetn3) encodes the calcium-binding p rotein, centrin, in the mouse. This work characterizes the Cetn2 gene. Stru cturally, Cetn2 consists of five exons and four introns, and contains a cla ssical TATA-less promoter. Cetn2 has two alternate transcription start site s, and a single length 3' untranslated region. Fluorescence in situ hybridi zation demonstrates that Cetn2 is an X-linked gene whose alleles replicate asynchronously during S-phase. Cetn2 encodes a 172 amino acid protein, with a predicted molecular mass of 19,795 Da (pI = 4.71), that contains all of the defining characteristics of centrin. Northern blot analysis indicates t hat Cetn2 is ubiquitously expressed in the tissues of adult mice. RT-PCR sh ows that Cetn2 and Cetn3, but not Cetn1, are expressed in NIH 3T3 cells. Im munofluorescence microscopy demonstrates that mouse centrin 2 protein local izes to the region immediately surrounding the centrioles in the centrosome of NIH 3T3 cells. (C) 2001 Elsevier Science B.V. All rights reserved.