The human genome contains hundreds or. repeats of the 3.3 kb family in regi
ons associated with heterochromatin. We have previously isolated a 3.3 kb-l
ike cDNA encoding a double homeodomain protein (DUX1). Demonstration that t
he protein was expressed in human rhabdomyosarcoma TE671 cells, and charact
erization of a homologous promoter suggested that functional DUX genes migh
t be present in 3.3 kb elements. In the present study, we describe two near
ly identical 3.3 kb/DUX genes derived from PAC 137F16 (DUX3), and TE671 gen
omic DNA (DUX5), both mapping to all the acrocentric chromosomes. Their pro
moters harbor a GC and a TATAA box, and the open reading frame of the intro
nless structural part encodes two DUX proteins differing by alternative tra
nslation initiation. The shorter protein of the DUX5 gene is identical to D
UX1. Using a protein truncation lest, we could show that these two proteins
are encoded by total RNA, but not by poly (A)(+) RNA, from different human
tissues and cell lines. Our results indicate that active genes of unusual
structure are present in chromosome regions characterized by large amounts
of heterochromatic repetitive DNA. (C) 2001 Elsevier Science B.V. All right
s reserved.