Prognostic significance of serum p53 protein and p53 antibody in patients with surgical treatment for head and neck squamous cell carcinoma

Objectives. This study aims at investigating the prognostic values of serum p53 protein and anti-p53 antibody in patients undergoing surgical treatmen t for head and neck squamous cell carcinoma (HNSCC). Methods. Serum p53 protein and anti-p53 antibody concentrations were determ ined by an enzyme-linked immunosorbent assay (ELISA) in 75 patients with HN SCC and 28 healthy controls. In 28 patients, formalin-fixed tumor tissues w ere also available for immunohistochemical staining by an anti-p53 DO7 mono clonal antibody. The results were correlated with the clinico-pathologic pa rameters. Results. The mean preoperative serum concentration of p53 protein in patien ts with HNSCc was significantly higher than healthy controls (59.45 pg/mL v s 16.4 pg/mL, p = .007). Preoperative serum p53 antibody was present in 23 (31%) patients and was present in one healthy control. Eighteen (62%) tumor tissues showed p53 overexpression by immunohistochemistry. The presence of serum anti-p53 antibody before operation was associated with a significant ly higher incidence (65%) or nodal metastasis compared with 27% nodal metas tasis in patients with absence of serum anti-p53 antibody (p = .002). Conclusion. Preoperative serum p53 antibody was a significant prognostic fa ctor for nodal metastasis of HNSCC. (C) 2001 John Wiley & Sons, Inc.