Hepatitis C virus infection: Other biological fluids than blood may be responsible for intrafamilial spread

Background/Aims: Several epidemiological studies have shown the existence o f other routes of transmission of the hepatitis C virus besides the parente ral one, but; the mechanisms involved are not yet understood. The general a im of this study was to evaluate the prevalence of hepatitis C virus infect ion in family contacts of infected patients and to analyze the possible ris k factors and alternative transmission routes. Methodology: One hundred and thirty-eight relatives of 45 patients tinder c ases) affected by C virus-related chronic hepatitis were studied. The relat ives were 45 spouses, 89 children and 4 cohabitants who underwent detection of serum anti-HCV antibodies; the anti-HCV-positive subjects were tested f or serum HCV-RNA. The index cases, all the spouses and only other infected relatives were tested for the presence of HCV-RNA in saliva Results: Antibodies to hepatitis C virus were detected in 5.7% of the famil y members while 11.1% of the analyzed spouses were serum HCV-RNA-positive. HCV-RNA was found in 44% of the examined saliva and 39% of these were found serum HCV-RNA-negative. The prevalence of hepatitis C virus among househol d contacts, excluding cases with previous parenteral exposure, was 3.6%. Conclusions: The epidemiological data on the intrafamilial spread of hepati tis C virus may be Reaction (PCR); underestimated owing to the existence of infected relatives serum-negative but saliva-positive for the presence of the virus. The whole of these observations suggests a possible role of biol ogical fluids in intrafamilial spread of hepatitis C virus.