The effect of granulocyte colony stimulating factor on the immune parameters in experimental obstructive jaundice

Background/Aims: Obstructive jaundice is an important clinical problem. It may cause complications such as renal insufficiency, cardiovascular sequels , coagulation defects, gastrointestinal bleeding, delayed wound healing, se condary biliary cirrhosis and sepsis. We investigated the effect of GM-CSF on immunological parameters in the experimental obstructive jaundice. Methodology: In our experimental study we studied four groups that consiste d of 28 rats. The 1st group consisted of sham rats, the 2nd group consisted of sham and GM-CSF applied rats and the 3rd and 4th groups consisted of ra ts that had obstructive jaundice. In the 4th group we applied 4 mug/kg GMCS F subcutaneously to the rats for 7 days. We measured the levels of neutroph ils, lymphocytes, leukocytes, interferon-alpha, CD32, CD34 and CD64 in all groups Results: In the jaundice group neutrophil, lymphocyte and leukocyte counts were found to be significantly lower compared to the other groups (P<0.005) . interferon-alpha and CD levels were found to be lower in the jaundice gro up compared to the other groups. In the GM-CSF applied jaundice group neutr ophils, lymphocytes, leukocytes and interferon-alpha levels were found to b e higher. CD34- CD64 levels were insignificantly increased in the GM-CSF gr oup whereas CD32 levels were significantly increased. Conclusions: We believe that in the prevention of serious septic complicati ons which have a high mortality risk, GM-CSF application to restore the mac rophage-neutrophil functions should be supported by advanced clinical studi es.