In this paper we present a new technique for data association using multias
signment for tracking a large number of closely spaced (and overlapping) ob
jects. The algorithm is illustrated on a biomedical problem, namely the tra
cking of a group of fibroblast (tissue) cells from an image sequence, which
motivated this work. Because of their proximity to one another and due to
the difficulties in segmenting the images accurately from a poor-quality Im
age sequence, the cells are effectively closely spaced objects (CSOs). The
algorithm presents a novel dichotomous, iterated approach to multiassignmen
t using successive one-to-one assignments of decreasing size with modified
costs. The cost functions, which are adjusted depending on the "depth" of t
he current assignment level and on the tracking results, are derived. The r
esulting assignments are used to form, maintain and terminate tracks with a
modified version of the probabilistic data association (PDA) filter, which
can handle the contention for a single measurement among multiple tracks i
n addition to the association of multiple measurements to a single track. E
stimation results are given and compared with those of the standard 2-dimen
sional one-to-one assignment algorithm. It is shown that iterated multiassi
gnment results in superior measurement-to-track association. The algorithms
presented here can be used for other general tracking problems, including
dense air traffic surveillance and control.