Simultaneous determination of guanosine and 8-hydroxy-guanosine by differential pulse voltammetry

Differential pulse voltammetry has been used for the simultaneous determina tion of guanosine and 8-hydroxyguanosine in phosphate buffers. Well-separat ed differential pulse voltammetric peaks for guanosine and 8-hydroxyguanosi ne are observed at pyrolytic graphite electrode. The proposed method is sim ple, rapid and provides a wide determination range of 0.5 muM to 0.1 mM wit h good reproducibility. Interference of ascorbic acid and some guanine rela ted compounds has also been studied and it is found that they do not interf ere at very low concentration level.